2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Zat standar sing digunakake ing kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen lan peralatan
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Sakabèhé, proporsi asam amino ing produk Sustar luwih dhuwur tinimbang ing produk Zinpro.
Bagean 8 Efek panggunaan
Efek saka macem-macem sumber mineral renik marang kinerja produksi lan kualitas endhog pitik petelur ing periode petelur pungkasan
Proses Produksi
Teknologi khelasi sing ditargetake
Teknologi emulsifikasi geser
Teknologi semprotan & pengeringan tekanan
Teknologi pendinginan & dehumidifikasi
Teknologi kontrol lingkungan sing luwih maju
Lampiran A: Metode kanggo Nemtokake distribusi massa molekul relatif peptida
Adopsi standar: GB/T 22492-2008
1 Prinsip Tes:
Iki ditemtokake nganggo kromatografi filtrasi gel kinerja dhuwur. Yaiku, nggunakake pengisi berpori minangka fase diam, adhedhasar bedane ukuran massa molekul relatif komponen sampel kanggo pamisahan, sing dideteksi ing ikatan peptida saka dawa gelombang penyerapan ultraviolet 220 nm, nggunakake piranti lunak pangolahan data khusus kanggo nemtokake distribusi massa molekul relatif kanthi kromatografi filtrasi gel (yaiku, piranti lunak GPC), kromatogram lan datane diolah, diitung kanggo entuk ukuran massa molekul relatif peptida kedele lan rentang distribusi.
2. Reagen
Banyu eksperimen kudu memenuhi spesifikasi banyu sekunder ing GB/T6682, panggunaan reagen, kajaba kanggo pranata khusus, kudu murni sacara analitis.
2.1 Reagen kalebu asetonitril (murni sacara kromatografi), asam trifluoroasetat (murni sacara kromatografi),
2.2 Zat standar sing digunakake ing kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen lan peralatan
3.1 Kromatograf Cair Kinerja Tinggi (HPLC): stasiun kerja utawa integrator kromatografi nganggo detektor UV lan piranti lunak pangolahan data GPC.
3.2 Unit filtrasi lan degassing vakum fase gerak.
3.3 Neraca elektronik: nilai ukur 0.000 1g.
4 Langkah operasi
4.1 Kondisi kromatografi lan eksperimen adaptasi sistem (kondisi referensi)
- 4.1.1 Kolom kromatografi: TSKgelG2000swxl300 mm×7,8 mm (diameter njero) utawa kolom gel liyané sing padha jinisé kanthi kinerja sing padha sing cocog kanggo nemtokaké protein lan peptida.
- 4.1.2 Fase gerak: Asetonitril + banyu + asam trifluoroasetat = 20 + 80 + 0,1.
- 4.1.3 Dawane gelombang deteksi: 220 nm.
- 4.1.4 Laju aliran: 0,5 mL/menit.
- 4.1.5 Wektu deteksi: 30 menit.
- 4.1.6 Volume injeksi sampel: 20μL.
- 4.1.7 Suhu kolom: suhu ruangan.
- 4.1.8 Supaya sistem kromatografi bisa nyukupi syarat deteksi, ditemtokake manawa miturut kahanan kromatografi ing ndhuwur, efisiensi kolom kromatografi gel, yaiku, jumlah pelat teoritis (N), ora kurang saka 10000 sing diitung adhedhasar puncak standar tripeptida (Glycine-Glycine-Glycine).
- 4.2 Produksi kurva standar massa molekul relatif
- Larutan standar peptida massa molekul relatif sing beda-beda ing ndhuwur kanthi konsentrasi massa 1 mg/mL disiapake kanthi pencocokan fase gerak, dicampur kanthi proporsi tartamtu, banjur disaring liwat membran fase organik kanthi ukuran pori 0,2 μm ~ 0,5 μm lan diinjeksi menyang sampel, banjur kromatogram standar dipikolehi. Kurva kalibrasi massa molekul relatif lan persamaane dipikolehi kanthi nggambar logaritma massa molekul relatif nglawan wektu retensi utawa kanthi regresi linier.
4.3 Perawatan sampel
Timbang kanthi akurat 10mg sampel ing labu ukur 10mL, tambahake sethithik fase gerak, kocok ultrasonik sajrone 10 menit, supaya sampel larut lan dicampur kanthi lengkap, diencerake karo fase gerak nganti tekan timbangan, banjur disaring liwat membran fase organik kanthi ukuran pori 0,2μm ~ 0,5μm, lan filtrat dianalisis miturut kahanan kromatografi ing A.4.1.
- 5. Pitungan distribusi massa molekul relatif
- Sawisé nganalisis larutan sampel sing disiapake ing 4.3 miturut kondisi kromatografi 4.1, massa molekul relatif sampel lan rentang distribusine bisa dipikolehi kanthi ngganti data kromatografi sampel menyang kurva kalibrasi 4.2 nganggo piranti lunak pangolahan data GPC. Distribusi massa molekul relatif saka peptida sing beda-beda bisa diitung nganggo metode normalisasi area puncak, miturut rumus: X=A/A total×100
- Ing rumus: X - Fraksi massa peptida massa molekul relatif ing total peptida ing sampel, %;
- A - Jembar puncak peptida massa molekul relatif;
- Total A - jumlah area puncak saben peptida massa molekul relatif, diitung nganti siji angka desimal.
- 6 Kabisaan Diulang
- Bentenane absolut antarane rong panentu independen sing dipikolehi miturut kahanan pengulangan ora kena ngluwihi 15% saka rata-rata aritmatika saka rong panentu kasebut.
- Lampiran B: Metode kanggo Nemtokake Asam Amino Bebas
- Adopsi standar: Q/320205 KAVN05-2016
- 1.2 Reagen lan bahan
- Asam asetat glasial: murni sacara analitis
- Asam perklorat: 0,0500 mol/L
- Indikator: indikator kristal violet 0,1% (asam asetat glasial)
- 2. Nemtokake asam amino bebas
Sampel dikeringake ing suhu 80°C sajrone 1 jam.
Lebokake sampel ing wadhah garing supaya adhem kanthi alami nganti suhu ruangan utawa adhemake nganti suhu sing bisa digunakake.Timbang kurang luwih 0,1 g sampel (akurat nganti 0,001 g) menyang labu kerucut garing 250 mL.Cepet nerusake menyang langkah sabanjure supaya sampel ora nyerep kelembapan sekitarTambahna 25 mL asam asetat glasial lan aduk rata ora luwih saka 5 menit.Tambahna 2 tetes indikator kristal unguTitrasi nganggo larutan titrasi standar 0,0500 mol/L (±0,001) asam perklorat nganti larutan kasebut owah saka ungu menyang titik pungkasan.
Cathet volume larutan standar sing dikonsumsi.
- Lakoni tes kosong ing wektu sing padha.
- 3. Pitungan lan asil
- Kandungan asam amino bebas X ing reagen kasebut dinyatakake minangka fraksi massa (%) lan diitung miturut rumus: X = C × (V1-V0) × 0,1445/M × 100%, ing rumus iki:
- C - Konsentrasi larutan asam perklorat standar ing mol saben liter (mol/L)
- V1 - Volume sing digunakake kanggo titrasi sampel nganggo larutan asam perklorat standar, ing mililiter (mL).
- Vo - Volume sing digunakake kanggo titrasi blanko nganggo larutan asam perklorat standar, ing mililiter (mL);
M - Massa sampel, ing gram (g).
| 0,1445: Massa rata-rata asam amino sing padha karo 1,00 mL larutan asam perklorat standar [c (HClO4) = 1,000 mol / L]. | 4.2.3 Larutan titrasi standar cerium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapake miturut GB/T601. | |
| Adopsi standar: Q/70920556 71-2024 | 1. Prinsip penentuan (Fe minangka conto) | Kompleks asam amino wesi nduweni kelarutan sing sithik banget ing etanol anhidrat lan ion logam bebas larut ing etanol anhidrat, bedane kelarutan antarane loro ing etanol anhidrat digunakake kanggo nemtokake tingkat khelasi kompleks asam amino wesi. |
| Ing rumus: V1 - volume larutan standar cerium sulfat sing dikonsumsi kanggo titrasi larutan uji, mL; | Etanol anhidrat; liyane padha karo klausul 4.5.2 ing GB/T 27983-2011. | 3. Langkah-langkah analisis |
| Lakoni rong uji coba kanthi paralel. Timbang 0,1 g sampel sing wis dikeringake ing suhu 103 ± 2 ℃ sajrone 1 jam, kanthi akurasi nganti 0,0001 g, tambahake 100 mL etanol anhidrat kanggo nglarutake, saring, sisa saring dicuci nganggo 100 mL etanol anhidrat paling ora kaping telu, banjur pindhah residu menyang labu kerucut 250 mL, tambahake 10 mL larutan asam sulfat miturut klausa 4.5.3 ing GB/T27983-2011, banjur tindakake langkah-langkah ing ngisor iki miturut klausa 4.5.3 "Panaskan nganti larut banjur adhemake" ing GB/T27983-2011. Lakoni uji coba kosong bebarengan. | 4. Nemtokake kandungan wesi total | 4.1 Prinsip panentu padha karo klausul 4.4.1 ing GB/T 21996-2008. |
4.2. Reagen & Larutan
| 4.2.1 Asam campuran: Tambahake 150mL asam sulfat lan 150mL asam fosfat menyang 700mL banyu lan aduk rata. | 4.2.2 Larutan indikator natrium difenilamin sulfonat: 5g/L, disiapake miturut GB/T603. | 4.2.3 Larutan titrasi standar cerium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapake miturut GB/T601. | |
| 4.3 Langkah-langkah analisis | Lakoni rong uji coba kanthi paralel. Timbang 0,1 g sampel, kanthi akurasi nganti 020001 g, lebokake ing labu kerucut 250 mL, tambahake 10 mL asam campuran, sawise larut, tambahake 30 ml banyu lan 4 tetes larutan indikator natrium dianilin sulfonat, banjur tindakake langkah-langkah ing ngisor iki miturut klausa 4.4.2 ing GB/T21996-2008. Lakoni uji blanko bebarengan. | 4.4 Representasi asil | Kandungan wesi total X1 saka kompleks wesi asam amino miturut fraksi massa wesi, nilai sing ditulis ing %, diitung miturut rumus (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - larutan standar cerium sulfat sing dikonsumsi kanggo titrasi larutan blanko, mL; | V0 - larutan standar cerium sulfat sing dikonsumsi kanggo titrasi larutan blanko, mL; | C - Konsentrasi nyata larutan standar cerium sulfat, mol/L5. Pitungan kandungan wesi ing kelatKandungan wesi X2 ing kelat miturut fraksi massa wesi, nilai sing ditulis ing %, diitung miturut rumus: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| Ing rumus: V1 - volume larutan standar cerium sulfat sing dikonsumsi kanggo titrasi larutan uji, mL; | V2 - larutan standar cerium sulfat sing dikonsumsi kanggo titrasi larutan blanko, mL;nom1-Massa sampel, g. Njupuk rata-rata aritmatika saka asil panentu paralel minangka asil panentu, lan bedane absolut saka asil panentu paralel ora luwih saka 0,3%. | 0,05585 - massa wesi fero sing ditulis ing gram padha karo 1,00 mL larutan standar cerium sulfat C[Ce(SO4)2,4H20] = 1,000 mol/L.nom1-Massa sampel, g. Njupuk rata-rata aritmatika saka asil panentu paralel minangka asil panentu, lan bedane absolut saka asil panentu paralel ora luwih saka 0,3%. | 6. Pitungan tingkat khelasiLaju khelasi X3, nilaine ditulis ing %, X3 = X2/X1 × 100Lampiran C: Cara kanggo Nemtokake Laju Khelasi Zinpro |
Adopsi standar: Q/320205 KAVNO7-2016
1. Reagen lan bahan
a) Asam asetat glasial: murni sacara analitis; b) Asam perklorat: 0,0500mol/L; c) Indikator: indikator kristal violet 0,1% (asam asetat glasial)
2. Nemtokake asam amino bebas
2.1 Sampel dikeringake ing suhu 80°C sajrone 1 jam.
2.2 Lebokake sampel ing wadhah garing supaya adhem kanthi alami nganti suhu ruangan utawa adhemake nganti suhu sing bisa digunakake.
2.3 Timbang kurang luwih 0,1 g sampel (akurat nganti 0,001 g) menyang labu kerucut garing 250 mL
2.4 Cepet nerusake menyang langkah sabanjure supaya sampel ora nyerep kelembapan sekitar.
2.5 Tambahake 25mL asam asetat glasial lan aduk rata ora luwih saka 5 menit.
2.6 Tambahna 2 tetes indikator kristal ungu.
2.7 Titrasi nganggo larutan titrasi standar 0,0500mol/L (±0,001) asam perklorat nganti larutan kasebut owah saka ungu dadi ijo sajrone 15 detik tanpa ngganti warna minangka titik pungkasan.
2.8 Cathet volume larutan standar sing dikonsumsi.
2.9 Lakoni tes kosong ing wektu sing padha.
- 3. Pitungan lan asil
- Basa Katalan
- Physicochemical parameters
V1 - Volume sing digunakake kanggo titrasi sampel nganggo larutan asam perklorat standar, ing mililiter (mL).
Vo - Volume sing digunakake kanggo titrasi blanko nganggo larutan asam perklorat standar, ing mililiter (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Alamat: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sichuan Province, China
Telpon: 86-18880477902
Produk
Mineral anorganik
- Mineral organik
- Basa Swahili
- Layanan khusus
- Pranala cepet
Profil Perusahaan
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Basa Gujarati | Klik kanggo pitakon | © Hak Cipta - 2010-2025: Kabeh Hak Dilindhungi Undhang-undhang. | Peta Situs PANELUSURAN UTAMA Telpon |
| Telp. | 86-18880477902 | Wong Jawa | Email |
| 8618880477902 | Cina | Prancis | |
| Bird | Cina | Prancis | Jerman Spanyol |
| Aquatic animals | Jepang | Korea | Basa Arab Yunani |
| Turki | Italia | ||
| Ruminant animal g/head day | January 0.75 | Basa Indonesia Wong Afrika Swedia |
Polandia
- Basa Basque
- Basa Katalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Basa Bulgaria
- Basa Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroasia
Walanda
| Application object | Basa Urdu Wong Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Basa Gujarati | Wong Haiti | Hausa | Kinyarwanda Wong Hmong Wong Hongaria |
| Piglets and fattening pigs | Igbo | Wong Jawa | Basa Kannada Khmer Wong Kurdi |
| Kirgiz | Latin | ||
| Bird | 300～400 | 45～60 | Makedonia Melayu Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Basa Norwegia
- Basa Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Basa Serbia
Basa Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Basa Swahili
Wong Tajik
Tamil
Telugu
Wong Thailand
| Application object | Basa Urdu Wong Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Basa Yiddish | Yoruba | Zulu | Kinyarwanda Oriya Wong Turkmen |
| Uighur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
